Reactive mesothelial cells are frequently found in the smear, of body fludid effusion
when associated with liver cirrhosis, pulmonary tuberculosis, congestive heart failure or
pulmonary infarction. But it is not easy to differentiate these reactive mesothelial cells
from macrophages or various types of malignant cells.
In order to investigate the morphology of hyperplastic mesothelial cells, in comparison
with the various types of malignant cells in the effusion, this experiment was designed.
The tissue reaction was evoked by intraperitoneal injection of 2.5㎖ of 2% Talcum
suspension.
The results obtained were as follows :
1) On light microscopic observation of the tissue, until 5 days, cytoplasm and nucleus
of misothelial cells became larger and nucleus became prominent. After then, cytoplasm
and nucleus became smaller and at 28 days after injection of Talcum suspension,
Cellular features became similar to those of normal ones.
2) On cytologic observation of touch print specimens, hyperplastic mesothelial cells
become round to oval, and their cytoplasm became conspicuous. Nuclei were vesicular,
and nucleoli wore prominent. N/C ratio was decreased than normal.
3) On electron microscopic observation, hyperplastic mesothelial cells did not haute
microvilli, but had a few cytoplasmic globular projections, loosely approximated cellular
junctions, larger and more abundant intracytoplaomic vesicles when compared with
normal one. Chromatic clumping, irregular thickening of nuclear membrane and
prominent nucleoli wore also nosed.
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